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FlowCam® 8100/8400

The FlowCam® 8000 series

The FlowCam 8000 series includes theFlowCam 8100, FlowCam 8400, FlowCam Cyanoand FlowCam LO.

The FlowCam 8100 is the most popular instrument of the Yokogawa Fluid Imaging Technologies product range.

This advanced dynamic image particle analysis system delivers high resolution pictures, over 40 morphological parameters as well as precise counts and sizes of the measured particles. 

It offers a very convenient operation with features such as automated focusing, automated sample processing and a self-cleaning program. It is configurable to individual needs:

  • Interchangeable Objectives with 2-,4-,10- and 20-times magnification for a measurement spectrum of 2µm to 1mm.
  • Monochrome or color CMOS-camera with a resolution of 1920x1200 pixels and a framerate of up to 60 frames per second (recommended 60 fps, maximum 100 fps).

The FlowCam 8400 provides all features of the 8100, but is equipped with an additional laser, which allows for 2-channel fluorescence measurements. Three excitation lasers with different wavelengths are available and are combined with two fluorescence detectors:

  • 488 nm laser - Ch 1: 570 nm long pass (Nile Red) / Ch 2: 520 nm ± 10 nm (FITC)
  • 488 nm laser - Ch 1: 650 nm long pass (Chlorophyll) / Ch 2: 525 nm ± 15 nm (FITC)
  • 532 nm laser - Ch 1: 650 nm long pass (Chlorophyll) / Ch 2: 575 nm ± 30 nm (Phycoerythrin)
  • 633 nm laser - Ch 1: 700 nm ± 10 nm (Chlorophyll) / Ch 2: 650 nm ± 10 nm (Phycocyanin)

The VisualSpreadsheet® software, included with every FlowCam, enables automatic particle characterization for sub-micron particles, using >40 morphological parameters. Create custom libraries for the different types of particles you encounter and let the image recognition software automatically group your measured particles and provide you with statistics, such as concentrations and particle counts. 

 

Key features of the FlowCam 8000 series

  • Automated particle characterization of microparticles (2µm to 1mm)
  • Interchangeable objectives and flow cells
  • Fluorescence Detection (8400 only)
  • >40 morphological measurements for each particle
  • High resolution images
  • Measure >10.000 particles per minute
  • Self-cleaning program

 

Applications

  • Biopharmaceuticals
  • Industry & Manufacturing
  • Harmful Algal Bloom Monitoring
  • Microalgae Cultivation
  • Phytoplankton & Zooplankton Research

 

FlowCam 8000 Series Specifications

Particle Size Range 2 µm to 1 mm
Magnification 20X (~200X total magnification) + corresponding flow cell
10X (~100X total magnification) + corresponding flow cell
4X (~40X total magnification) + corresponding flow cell
2X (~20X total magnification) + corresponding flow cell
Sample Processing Capability 0.05 mL/minute at 20X and up to 5 mL/minute at 2X
Camera High resolution (1920x1200 pixels) CMOS. Monochrome and color available.
Frame Rate recommended maximum 60 fps, maximum 100 fps
Focus system Autofocus
Fluidics Micro syringe pump with multiple sizes to optimize flow rates: 0.5 mL, 1 ml, 5 ml, 12.5 ml
Data Acquisition Method FlowCam 8400 - fluorescence based laser triggering and flow imaging
FlowCam 8100 - flow imaging
Fluorescence Emission & Detection Excitation Options (488 nm, 532 nm, 633 nm) with 2-Channel Fluorescence Detection:
- 488 nm laser - Ch 1: 570 nm long pass (Nile Red) / Ch 2: 520 nm ± 10 nm (FITC)
- 488 nm laser - Ch 1: 650 nm long pass (Chlorophyll) / Ch 2: 525 nm ± 15 nm (FITC)
- 532 nm laser - Ch 1: 650 nm long pass (Chlorophyll) / Ch 2: 575 nm ± 30 nm (Phycoerythrin)
- 633 nm laser - Ch 1: 700 nm ± 10 nm (Chlorophyll) / Ch 2: 650 nm ± 10 nm (Phycocyanin)
VisualSpreadsheet® Interactive, image-based analytical software that generates 40+ particle measurements per cell. Filter, sort, and classify data based on user-defined criteria. Create libraries to automate classification for future sample analyses.
Included Accessories Integrated Windows PC with VisualSpreadsheet® software, mouse and keyboard, Monitor
Device Dimensions H x L x W: 38 cm x 45 cm x 37 cm